|Attachment and biofilm formation on stainless steel by Escherichia coli O157H7 as affected by curli production|
|Year of publication||2004년 이전|
|Author||J. H. Ryu., H. Kim., J.F. Frank., & L.R. Beuchat|
|Publication in journal||Letters in Applied Microbiology 2004, 39, 359–362|
|Status of publication||Published|
Aims: The aim of this study was to determine the role of curli in attachment and bioﬁlm formation by Escherichia coli O157:H7 on stainless steel.
Methods and Results: Three curli-deﬁcient strains (43895), 43894) and E0018)) and three curli over-producing strains (43895+, 43894+ and E0018+) of E. coli O157:H7 were studied. Stainless steel coupons (SSC) were immersed in cell suspensions of each strain for 24 h at 4°C. The number of cells attached to SSC was determined. To determine the ability of attached cells to form bioﬁlm, SSC were immersed in 10% of tryptic soya broth up to 6 days at 22°C. Curli-deﬁcient and curli-producing strains did not differ in their ability to attach to SSC, but only curli-producing strains formed bioﬁlms.
Conclusions: Curli production by E. coli O157:H7 does not affect attachment of cells on stainless steel but curli-producing strains are better able to form bioﬁlms.
Signiﬁcance and Impact of the Study: Curli production by E. coli O157:H7 enhances its ability to form bioﬁlm on stainless steel, thereby potentially resulting in increased difﬁculty in removing or killing cells by routine cleaning and sanitizing procedures used in food-processing plants.