Korea University Food Microbiology Laboratory

Attachment and biofilm formation on stainless steel by Escherichia coli O157H7 as affected by curli production
Year of publication 2004년 이전
Author J. H. Ryu., H. Kim., J.F. Frank., & L.R. Beuchat
Publication in journal Letters in Applied Microbiology 2004, 39, 359–362
Status of publication Published


Aims:   The  aim  of  this  study  was  to  determine  the  role  of  curli  in  attachment  and  biofilm  formation  by  Escherichia coli  O157:H7  on  stainless  steel.

Methods and Results:   Three curli-deficient strains (43895), 43894) and E0018)) and three curli over-producing strains  (43895+,  43894+  and  E0018+)  of  E.  coli  O157:H7  were  studied.  Stainless  steel  coupons  (SSC)  were immersed  in  cell  suspensions  of  each  strain  for  24  h  at  4°C.  The  number  of  cells  attached  to  SSC  was  determined. To  determine  the  ability  of  attached  cells  to  form  biofilm,  SSC  were  immersed  in  10%  of  tryptic  soya  broth  up  to 6  days  at  22°C.  Curli-deficient  and  curli-producing  strains  did  not  differ  in  their  ability  to  attach  to  SSC,  but only  curli-producing  strains  formed  biofilms. 

Conclusions:   Curli  production  by  E.  coli  O157:H7  does  not  affect  attachment  of  cells  on  stainless  steel  but curli-producing  strains  are  better  able  to  form  biofilms. 

Significance and Impact of the Study: Curli production by E. coli O157:H7 enhances its ability to form biofilm on stainless steel, thereby potentially resulting in increased difficulty in removing or killing cells by routine cleaning and sanitizing procedures used in food-processing plants.